Recombinant Alkali-tolerant Protein A (rat-PA)

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  • SKU: GEN-NRPA08S
  • FORMULATION: Lyophilized from a sterile solution of 5 mM PB (4 mM Na2HPO4, 1 mM NaH2PO4, pH 7.4)
  • MOL. WEIGHT: 31.2 kDa (approximately 28 kDa on SDS-PAGE)
  • THEORETICAL PI: 5.24
  • A280NM (1mg / ml): 0.191
  • RESOURCES: Escherichia coli (E. coli)
  • PURITY: ≥95%
  • ACID RESISTANCE: > 0.1 M HCl
  • RESISTANCE TO ALCALI: > 0.1 M NaOH
  • HEAT RESISTANCE: 60 ° C (one hour)
  • STORAGE STATE: -20 ° C
  • STORAGE PERIOD: 3 years

Protein A is a cell wall protein derived from Staphylococcus aureus that exhibits unique binding properties for IgG from a variety of mammalian species and also for certain IgM and IgA. It binds to the Fc region of immunoglobulins preferably through interaction with the heavy chain.

It is conjugated to a wide variety of reporter molecules, including fluorescent dyes, enzyme markers, biotin, colloidal gold, and radioactive iodine for use in immunology and other biological research without affecting the antibody binding site. rat-PA has improved protein stability and is alkali tolerant by replacing residues.

It is also immobilized on solid matrices such as agarose beads for the purification of IgG from ascetic fluid, serum, culture supernatants, cell extracts, or proteins of interest. For efficient covalent conjugation with the resin, a cysteine ​​residue is inserted into the C-terminus of rat-PA.

Notes: It is recommended that the product be reconstituted with sterile water to a final concentration of 10 mg/ml. newly.

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