GnpIS is an information repository for plant phenomics that shops entire subject and greenhouse experimental knowledge together with setting measures. It permits long-term entry to datasets following the FAIR ideas: Findable, Accessible, Interoperable, and Reusable, through the use of a versatile and unique strategy. It relies on a generic and ontology pushed knowledge mannequin and an modern software program structure that uncouples knowledge integration, storage, and querying.
It takes benefit of worldwide requirements together with the Crop Ontology, MIAPPE, and the Breeding API. GnpIS permits dealing with knowledge for a variety of species and experiment varieties, together with multiannual perennial vegetation experimental community or annual plant trials with both uncooked knowledge, i.e., direct measures, or computed traits. It additionally ensures the combination and the interoperability amongst phenotyping datasets and with genotyping knowledge. This is achieved by means of a cautious curation and annotation of the important thing sources performed in shut collaboration with the communities offering knowledge.
Our repository follows the Open Science knowledge publication ideas by making certain citability of every dataset. Finally, GnpIS compliance with worldwide requirements allows its interoperability with different knowledge repositories therefore permitting knowledge hyperlinks between phenotype and different knowledge sorts. GnpIS can subsequently contribute to rising worldwide federations of knowledge programs. A plant develops the dynamic phenotypes from the interplay of the plant with the setting. Understanding these processes that span plant’s lifetime in a completely altering setting is crucial for the development of primary plant science and its translation into software together with breeding and crop administration. The plant analysis group was thus confronted with the necessity to precisely measure numerous traits of an more and more massive variety of vegetation to assist vegetation to adapt to resource-limiting setting and low-input agriculture. In this overview, we define the event of plant phenotyping as a multidisciplinary subject.
The Neonicotinoid Insecticide Imidacloprid Disrupts Bumblebee Foraging Rhythms and Sleep
Neonicotinoids have been implicated in the massive declines noticed in bugs similar to bumblebees, an necessary group of pollinators. Neonicotinoids are agonists of nicotinic acetylcholine receptors which can be discovered all through the insect central nervous system and are the primary mediators of synaptic neurotransmission. These receptors are necessary for the operate of the insect central clock and circadian rhythms. The clock permits pollinators to coincide their exercise with the provision of floral sources and favorable flight temperatures, in addition to influence studying, navigation, and communication.
Here we present that publicity to the field-relevant focus of 10 μg/L imidacloprid brought on a discount in bumblebee foraging exercise, locomotion, and foraging rhythmicity. Foragers confirmed a rise in daytime sleep and a rise in the proportion of exercise occurring at evening. This might scale back foraging and pollination alternatives, decreasing the flexibility of the colony to develop and reproduce, endangering bee populations and crop yields. The genus Lolium includes many species, of which L. perenne ssp. multiflorum, L. perenne ssp. perenne, and L. rigidum are of worldwide agricultural significance as each pasture crops and as weeds. These three species are inter-fertile, obligate out-crossers with a self-incompatible copy system. This mixture contributes to excessive genetic range that provides new variants throughout enlargement to new pure areas and agricultural environments.
Human dispersal, de-domestication and crop-weed hybridization occasions between Lolium spp., or with others similar to Festuca spp., are possible related to their distinct weediness skills. Furthermore, new introductions adopted by introgression could hasten adaptation to new environments.
Applying FAIR Principles to Plant Phenotypic Data Management in GnpIS
Supply chain and environmental evaluation of the important oil manufacturing utilizing Calendula ( Calendula Officinalis) as uncooked materials
Biomass has been thought-about a possible supply of value-added merchandise and vitality vectors. Most biomass research have researched the perfect pathways or processes to improve this renewable uncooked materials by means of stand-alone processes or biorefineries. The biomass provide chain is an important facet in the financial evaluation of biomass upgrading since many of the uncooked supplies want to be transported. A provide chain evaluation offers an concept concerning the availability, actual prices, and storage situations of the uncooked materials to assure an correct feasibility evaluation and a standardized manufacturing course of. Calendula (Calendula Officinalis) is an fragrant plant used to produce useful extracts in the beauty and pharmaceutical industries. Nevertheless, excessive quantities of exhausted biomass (greater than 95% w/w) are produced and wasted.
Theseresidues symbolize an environmental challenge to be solved by means of the implementation of valorizing choices. This paper analyses the availability chain and environmental influence of important oil manufacturing utilizing Calendula (Calendula Officinalis) as a uncooked materials in the Colombian context. The case research includes a single-objective optimization of the calendula provide chain to produce important oil and the life cycle evaluation (LCA) of the method by means of a cradle-to-gate strategy in the Colombian context.
Custom Testing of Samples for Daclizumab (Zenapax) by ELISA
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, GE601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
PinPoint-HR System for Platform Cell Line Generation & Retargeting of AAVS1 Safe Harbor Locus (includes PIN410A-1, CAS601A-1, PIN200A-1, PIN510A-1, & PIN600A-1)
This Laboratory testing revealed lymphocytic choriomeningitis virus antibody ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.
Human Laboratory testing revealed lymphocytic choriomeningitis virus antibody(LCMV-Ab)ELISA Kit
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples for Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples for Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples for Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Adiponectin (ADP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples for Adiponectin (ADP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Adiponectin elisa. Alternative names of the recognized antigen: GBP28
ApM1
AdipoQ
Acrp30
ACDC
APM1
C1Q And Collagen Domain Containing
Adipocyte Complement-Related Protein Of 30 KDa
Adipose Most Abundant Gene Transcript 1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Adiponectin (ADP) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Pepsin (PP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Pepsin (PP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Pepsin (PP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Pepsin (PP) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Pepsin (PP) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Pepsin elisa. Alternative names of the recognized antigen: n/a
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Pepsin (PP) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Lymphotactin (Lptn) were tested on 3 different plates, 8 replicates in each plate
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Lymphotactin (Lptn) were tested on 3 different plates, 8 replicates in each plate
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Lymphotactin (Lptn) were tested on 3 different plates, 8 replicates in each plate
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Lymphotactin (Lptn) were tested on 3 different plates, 8 replicates in each plate
Known also as Lymphotactin elisa. Alternative names of the recognized antigen: XCL1
SCYC1
LTN
ATAC
SCM1-A
SCM1a
LPTN
Lymphotaxin
Chemokine C-Motif Ligand 1
Small Inducible Cytokine Subfamily C, Member 1
XC chemokine ligand 1
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Lymphotactin (Lptn) in samples from n/a with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Ghrelin (GHRL) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Ghrelin (GHRL) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Ghrelin (GHRL) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Ghrelin (GHRL) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Ghrelin (GHRL) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Ghrelin elisa. Alternative names of the recognized antigen: MTLRP
Growth Hormone-Releasing Peptide
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Ghrelin (GHRL) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mini Samples for Estrone (E1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mini Samples for Estrone (E1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mini Samples for Estrone (E1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Estrone (E1) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Competitive Enzyme-linked immunosorbent assay for detection of Mini Samples for Estrone (E1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Estrone elisa. Alternative names of the recognized antigen: Oestrone
Description: Enzyme-linked immunosorbent assay based on the Competitive Inhibition method for detection of Mini Samples Estrone (E1) in samples from Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Arginase (ARG) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Arginase (ARG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Arginase (ARG) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Arginase (ARG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Arginase (ARG) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Arginase (ARG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples Mouse Arginase (ARG) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples Mouse Arginase (ARG) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Known also as Arginase elisa. Alternative names of the recognized antigen: ARG1
Arginase I
Liver Arginase
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mini Samples Mouse Arginase (ARG) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.
The Intra-assay Precision is determined when 3 samples with low, middle and high level of Mini Samples for Lysozyme (LZM) were tested on 3 different plates, 8 replicates in each plate
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mini Samples for Lysozyme (LZM) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
The outcomes confirmed the perfect areas to improve Calendula in Colombia (i.e., Manizales and Bucaramanga), supplying 1.1 % of the whole product demand. The optimum product movement to prospects was 0.32 tons/yr, and the required feedstock from suppliers was 162 tons/yr. The agricultural stage of important oil manufacturing represented the best environmental influence of the availability chain. In explicit, plastic sheets, natural fertilizers, and chemical fungicides have been the primary contributors to this influence.