Vonwillabrand Factor Viii Binding Assay

Description: The Cereblon Binding Assay Kit (FP) is designed for testing and profiling of Cereblon (CRBN) inhibitors using Fluorescence Polarization. The assay is a competitive binding assay, based on the binding of fluorescently-labeled Thalidomide, a CRBN binder, to purified recombinant CRBN._x000D_ CRBN is the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 protein ligase complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins. CRBN is a direct protein target for the immunomodulatory and antiproliferative activities of thalidomide analogues such as lenalidomide and pomalidomide._x000D_ The Cereblon Binding Assay Kit (FP) comes in a convenient 96-well format, with enough purified CRBN, Cy5-labeled Thalidomide, and assay buffer for 100 enzyme reactions. In addition, the kit includes the CRBN inhibitor Pomalidomide for use as an inhibitor control._x000D_
The key to the CRBN Assay Kit is the fluorescently-labeled Thalidomide. Using this kit, only one simple step on a microtiter plate is required for CRBN reactions. The Cy5-labeled Thalidomide is incubated with a sample containing CRBN to produce a change in fluorescent polarization that can be measured using a fluorescence reader.

Description: The CytoSelect Cell Viability and Cytotoxicity Assay Kit provides a simple format for monitoring cell viability via metabolic activity. Live cells are detected with either MTT (colorimetric detection) or Calcein AM (fluorometric detection). Dead cells are detected by EthD-1 reagent (fluorometric). All 3 detection reagents are included, along with Saponin (a cell death initiator). Prior to the assay, cells may be treated with compounds or agents that affect cell viability. This kit is suitable for eukaryotic cells, not yeast or bacteria.

Description: This product includes 60 ul of 1000 x PSA dye for more than 200 assays with the assay volume of 0.3 ml using 96-well plates or cuvettes.

Description: The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can then be measured using a chemiluminescence reader.

Description: Minimum order quantity: 1 unit of 1KIT

Description: Purified Polyclonal Factor VIII antibody

Description: Coagulation Factor VIII (FVIII) is an essential blood-clotting protein. By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation Factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the F9a-dependent activation of F10. Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). It is tightly associated in the blood with VWF, which serves as a protective carrier protein for Factor VIII (Toole et al., 1984; Hoyer, 1994).

Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF594 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF594 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF680 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF680 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF770 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF770 conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF680R conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF680R conjugate, Concentration: 0.1mg/mL

Description: Primary antibody against von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) (F8/86),CF488A conjugate, Concentration: 0.1mg/mL